Chimie bioanalytique
- Cours (CM) -
- Cours intégrés (CI) -
- Travaux dirigés (TD) -
- Travaux pratiques (TP) -
- Travail étudiant (TE) -
Langue de l'enseignement : Français
Description du contenu de l'enseignement
- Purification of biomolecules using liquid chromatography (SEC, IEC, HIC, RP, HILIC, IMAC, affinity). Preparative and miniaturized scales.
- Purification of biomolecules using electrophoresis in denaturating or native conditions. (1D, 2D, off gel).
- Contribution of mass spectrometry to the characterization of biomolecules: ionization sources and analyzers for biomolecules, interpretation of mass spectra MS and MS/MS of biomolecules, identification of proteins from mass spectrometry data.
- Shotgun and targeted proteomic strategies.
- Strategies of relative and absolute quantification of proteins in complex mixtures.
- Supramolecular mass spectrometry.
Compétences à acquérir
- Understanding of the different approaches for the characterization and the quantification of biomolecules.
- Design of a convenient proteomic strategy.
Contact
Faculté de chimie
1, rue Blaise Pascal - BP 2029667008 STRASBOURG CEDEX
0368851672
Formulaire de contact
Responsable
Laurence Sabatier
