Enzymology practicals
- Cours (CM) 10h
- Cours intégrés (CI) -
- Travaux dirigés (TD) 8h
- Travaux pratiques (TP) 27h
- Travail étudiant (TE) -
Langue de l'enseignement : Anglais
Description du contenu de l'enseignement
Objectives for knowledge :
- Understanding and practicing enzymology basics including:
- Enzymatic activity
- Kinetics of enzymatic activity
- Immobilizing enzymes, a lecture and practice at small and medium scales
- The beta-galactosidase as a prototypic enzyme
- Hydrolysis of lactose, purified and contained in milk
- Discussions on the strategies and results
Compétences à acquérir
Objectives in terms of skills :
- Acquire a good practice for work with proteins including enzymes, with kinetics
- Using spectrophotometers
- Acquire the notion of making appropriate graphs, of experimental variability/reproducibility
- Be able to relate the theory of enzymology to experimental results
- Be able to analyze and discuss on experimental results
- Be able to present experimental results
Contact
Responsable
Claire Gaveriaux-Ruff
Bruno Chatton
