Objectives in terms of knowledge (lecture content):Objectives in terms of knowledge (lecture content):

• Introduction to the bases of biochemistry, the purification of different components (essentially proteins) by the use of chromatography technics.
• Technics used: affinity chromatography, ion exchange and size exclusion. Analysis of the purified proteins on SDS-PAGE gel, native gel and visualization of proteins by staining with Coomasie blue.
• Realization of the experiments according to the protocol provided and the information gleaned from the data sheets (under supervision of a teacher).

Course support ; Technical data sheets for different products and chromatography columns and protocos given by the teachers  

Objectives in terms of skills :
Ability to use various basic chromatography technics to purify proteins and amino acids as well as to analyze them.
 

Lecture PDF, Publications, protocoles, G healthcare documentations on chromatography technics. Technical sheets of the products used.